Opusculum physiologum & anatomicum in duos libellos distinctum: In quibus primùm, de integritatis & corruptionis virginum notis, deinde, de grauiditate & partu naturali mulierum in quo ossa pubis & ilium distrahi, dilucidè tractatur ....

In 1595 Pineau demonstrated the vestigial foramen ovale in the adult heart, settling the question of the perviousness of the septum of the heart. His work was first published in 1597. He published this study in a frank treatise on virginity and the ways of losing it. Digital facsimile from BnF Gallica at this link.