Electrophoresis of serum globulin. II- Electrophoretic analysis of normal and immune sera.

Tiselius showed that an antibody that he produced in a rabbit by immunizing it with albumin (ovalbumin) was clearly located in the gamma globulin fraction. He summarized the paper with this statement: "Investigation of a highly potent anti-egg albumin ('ovalbumin') serum from rabbit showed that the antibody function migrated with the gamma globulin fraction only."

Consequences of separation and identification of the antibody were that it could be studied and characterized, could be quantitated, and could be used to identify pathologic conditions in which it was either absent or low.

(Thanks to Juan Weiss for this reference and its interpretation.)