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Historical Bibliography Updated: July 8, 2022

The construction of molecular cloning vehicles. II. A multipurpose cloning system.

Publication Details

Gene, 2, 95-113. 1977 CE.

Order of authorship in the original publication Bolivar, Rodriguez, Betlach...Boyer...The authors describe the composition and molecular construction of pBR-322 (named after Bolivar and Rodriguez) and call it "the most versatile plasmid we have ever constructed." The paper includes the first restriction map of the plasmid. Plasmid pBR-322 was the plasmid used in the synthesis of the gene for somatostatin (No. 10964), leading to the biotechnological production of insulin by Boyer's biotechnology company, Genentech in 1977. 

The authors presented this paper in June 1977. It was published in the journal Gene in November, 1977. The paper also appeared in Beers, Basset (eds.) Recombinant molecules: Impact on science and society, Miles International Symposium, Series No. 10. New York: Raven Press, 1977. The book form version of the paper indicates that it was submitted for publication in the journal, suggesting that the version in book form might have been released before the publication in the journal.

(Thanks to Juan Weiss for this reference and its interpretation.)

Catalog MetadataReference Information
Entry Number#12031
Permanent Linkhttps://staging.historyofmedicine.com/entry/14239
Author Bio LinkWikipedia ↗
External URLthe-construction-of-molecular-cloning-vehicles-in-recombinant-moleculars-impact-on-science-and-society-miles-international-symposium-series-10-beers-basset-eds

Geographic Context

Mentioned in annotation: New York